|
KMID : 1007520140230061997
|
|
Food Science and Biotechnology
2014 Volume.23 No. 6 p.1997 ~ p.2005
|
|
|
Novel Bioconversion of Sodium Glutamate to γ-Amino Butyric Acid by Co-culture of Lactobacillus plantarum K154 in Ceriporia lacerata Culture Broth
|
|
Lee Eun-Ji
Lee Sam-Pin
|
|
|
Abstract
|
|
|
|
Co-cultivation was performed for optimization of microbial GABA production in Ceriporia lacerata cultures. C. lacerata was grown in a defined medium containing 3% glucose, 3% soybean flour, 0.15% MgSO4, and 5% rice bran for 7 days at 25oC in a shaking culture, resulting in production of a culture with 0.63% (w/v) acidity, 11.2 g/L of mycelia, 3.0 g/L of exopolysaccharides, 6.24 unit/mL of α-amylase, 45.6 unit/mL of protease and 4.1 unit/mL of cellulase. Viable cell counts of Lactobacillus plantarum K154 co-cultured in C. lacerata culture broth were increased by 1×1010 CFU/mL. In co-cultivation with L. plantarum K154, 15.53 mg/mL of GABA was produced from sodium glutamate. A 40 kDa molecular weight protein co-produced with GABA by L. plantarum K154 was confirmed to be GAPDH based on protein sequencing. Cocultivation of L. plantarum K154 in C. lacerata culture broth efficiently produced functional ingredients, including GABA, peptides, polysaccharides, and probiotics.
|
|
|
KEYWORD
|
|
|
sodium glutamate, γ-aminobutyric acid, Lactobacillus plantarum, Ceriporia lacerata, probiotic
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|
|